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    <p>In auto generated <code>OpeningFcn</code> callback, if you're using GUIDE You can define variables/fields in <code>handle</code> structure.</p> <p>This is initialization part. The data used by this can be shared across all widgets on the GUI.</p> <p>So, in this callback, for <code>variable_1</code>,<code>variable_2</code>, <code>sum</code>, etc. you can have something like following:</p> <pre><code>handles.variable_1 = 1; handles.variable_1 = 2; handles.sum = handles.variable_1 + handles.variable_1 = 1; % At end update handles structure handles.output = hObject; guidata(hObject, handles); </code></pre> <p>In other callback, say your <code>Button</code> or <code>Edit</code> callback</p> <p>you can update these values as normal like following</p> <pre><code>handles.variable_1 = 12; handles.variable_1 = 23; handles.sum = handles.variable_1 + handles.variable_1 = 1; % Now, at end of callback save the change you made to the structure guidata(hObject,handles) </code></pre> <p>To verify these values type <code>handles</code> at command window.</p> <p>Ref :<strong><em><a href="http://www.mathworks.in/help/matlab/ref/guidata.html" rel="nofollow">Store or retrieve GUI data</a></em></strong> for more details.</p> <p>To use these variables from GUI to other script simple assign them in workspace like <code>assignin('base','variable_1',handles.variable_1);</code> ,etc in any of the callback where <code>variable_1</code> is getting updated.</p> <p>Then, in your m-script you can directly use these variables.</p>
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    1. POGlobal variables set in GUI with no values - Matlab
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    1. COThank you for your reply. I know I can define the sum expression inside the GUI code, but that's not the real propouse. I'm sorry for not having made it clear. I have to pass those variables to the m-File, because the m-File will be much more complex than that.
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    2. CO@Luiz which `m-file` ? A `m-file` other than that of GUI itself ? GUI code, the `callbacks` will be in `m-file` and its design will be saved in a `.fig` file Have you created any other `m-file` by your own, other than that generated from `GUIDE` ?
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    3. COYes, the m-File is the one named "test" in my program, the one which makes the sum of the variables. So, the GUI pushbutton calls this "test.m" as shown above!
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