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    <p>Many thanks to help me out I went through AnalyzeSkeleton and got the result in SekeletonResult Response by Using IJ. for this I have used IJ.run(imp, "Skeletonize", "");</p> <pre><code> // Initialize AnalyzeSkeleton_ AnalyzeSkeleton_ skel = new AnalyzeSkeleton_(); skel.calculateShortestPath = true; skel.setup("", imp); // Perform analysis in silent mode // (work on a copy of the ImagePlus if you don't want it displayed) // run(int pruneIndex, boolean pruneEnds, boolean shortPath, ImagePlus origIP, boolean silent, boolean verbose) SkeletonResult skelResult = skel.run(AnalyzeSkeleton_.NONE, false, true, null, true, false); // Read the results Object shortestPaths[] = skelResult.getShortestPathList().toArray(); double branchLengths[] = skelResult.getAverageBranchLength(); int branchNumbers[] = skelResult.getBranches(); long totalLength = 0; for (int i = 0; i &lt; branchNumbers.length; i++) { totalLength += branchNumbers[i] * branchLengths[i]; } double cumulativeLengthOfShortestPaths = 0; for (int i = 0; i &lt; shortestPaths.length; i++) { cumulativeLengthOfShortestPaths +=(Double)shortestPaths[i]; } System.out.println("totalLength "+totalLength); System.out.println("cumulativeLengthOfShortestPaths "+cumulativeLengthOfShortestPaths); System.out.println("getNumOfTrees "+skelResult.getNumOfTrees()); System.out.println("getAverageBranchLength "+skelResult.getAverageBranchLength().length); System.out.println("getBranches "+skelResult.getBranches().length); System.out.println("getEndPoints "+skelResult.getEndPoints().length); System.out.println("getGraph "+skelResult.getGraph().length); System.out.println("getJunctions "+skelResult.getJunctions().length); System.out.println("getJunctionVoxels "+skelResult.getJunctionVoxels().length); System.out.println("getListOfEndPoints "+skelResult.getListOfEndPoints().size()); System.out.println("getListOfJunctionVoxels "+skelResult.getListOfJunctionVoxels().size()); System.out.println("getMaximumBranchLength "+skelResult.getMaximumBranchLength().length); System.out.println("getNumberOfVoxels "+skelResult.getNumberOfVoxels().length); System.out.println("getQuadruples "+skelResult.getQuadruples().length); this method .but I am not able to find which method in Skeleton Result class returns bifuraction point could you please help me little more thanks Amar </code></pre>
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    1. POBifurcation and ridge ending point
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    1. COHi jan Many thanks to help me out.one little more help I am able to get SkeletonResult class responcs by above implemented code.but which method in SkeletonResult class return Bifuraction and edning point.there is one method Endpoint in skeletonResult class but for every image it return only 1
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