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    <p>You can use <code>GridView1.Rows(0)</code> to get the first row:</p> <pre><code>Dim firstRow = GridView1.Rows(0) Dim TextBox2 = DirectCast(firstRow.FindControl("TextBox2"), TextBox) Dim DropDownList3 = DirectCast(firstRow.FindControl("DropDownList3"), DropDownList) If TextBox2.Text.Trim.Length = 0 OrElse DropDownList3.SelectedIndex = -1 Then label6.Text = "HospNo,Date,SurgeryCode/Method are Mandatory" End If </code></pre> <p>But this is not the approach you should use in ASP.NET. Instead use <a href="http://msdn.microsoft.com/en-us/library/system.web.ui.webcontrols.requiredfieldvalidator%28v=vs.100%29.aspx" rel="nofollow"><code>RequiredFieldValidator</code></a> which can ven validate on client-side.</p> <p>For example:</p> <pre><code>&lt;asp:GridView ID="GridView1" OnSelectedIndexChanged="Grid1_SelectedIndexChanged" AutoGenerateSelectButton="true" AutoGenerateColumns="false" runat="server" Width="300"&gt; &lt;RowStyle CssClass="GridViewRowStyle" /&gt; &lt;AlternatingRowStyle CssClass="GridViewAlternatingRowStyle" /&gt; &lt;HeaderStyle CssClass="GridViewHeaderStyle" /&gt; &lt;SelectedRowStyle BackColor="Aqua" /&gt; &lt;Columns&gt; &lt;asp:TemplateField HeaderText="Column 1" HeaderStyle-HorizontalAlign="Left"&gt; &lt;ItemTemplate&gt; &lt;asp:TextBox runat="server" ID="TxtColumn1" Text='&lt;%# Bind("Column1") %&gt;'&gt;&lt;/asp:TextBox&gt; &lt;asp:RequiredFieldValidator id="RequiredFieldValidator1" EnableClientScript="true" ControlToValidate="TxtColumn1" Display="Static" ErrorMessage="HospNo,Date,SurgeryCode/Method are Mandatory" runat="server"/&gt; &lt;/ItemTemplate&gt; &lt;/asp:TemplateField&gt; &lt;/Columns&gt; &lt;/asp:GridView&gt; </code></pre>
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