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    <p>Try this:</p> <pre><code>USBSerialNumber usb = new USBSerialNumber(); string serial = usb.getSerialNumberFromDriveLetter("f:\"); MessageBox.Show(serial); </code></pre> <p>Here's the internals for the USBSerialNumber class:</p> <pre><code>using System; using System.Collections.Generic; using System.Text; using System.Management; namespace USBDriveSerialNumber { public class USBSerialNumber { string _serialNumber; string _driveLetter; public string getSerialNumberFromDriveLetter(string driveLetter) { this._driveLetter = driveLetter.ToUpper(); if(!this._driveLetter.Contains(":")) { this._driveLetter += ":"; } matchDriveLetterWithSerial(); return this._serialNumber; } private void matchDriveLetterWithSerial() { string[] diskArray; string driveNumber; string driveLetter; ManagementObjectSearcher searcher1 = new ManagementObjectSearcher("SELECT * FROM Win32_LogicalDiskToPartition"); foreach (ManagementObject dm in searcher1.Get()) { diskArray = null; driveLetter = getValueInQuotes(dm["Dependent"].ToString()); diskArray = getValueInQuotes(dm["Antecedent"].ToString()).Split(','); driveNumber = diskArray[0].Remove(0, 6).Trim(); if(driveLetter==this._driveLetter){ /* This is where we get the drive serial */ ManagementObjectSearcher disks = new ManagementObjectSearcher("SELECT * FROM Win32_DiskDrive"); foreach (ManagementObject disk in disks.Get()) { if (disk["Name"].ToString() == ("\\\\.\\PHYSICALDRIVE" + driveNumber) &amp; disk["InterfaceType"].ToString() == "USB") { this._serialNumber = parseSerialFromDeviceID(disk["PNPDeviceID"].ToString()); } } } } } private string parseSerialFromDeviceID(string deviceId) { string[] splitDeviceId = deviceId.Split('\\'); string[] serialArray; string serial; int arrayLen = splitDeviceId.Length-1; serialArray = splitDeviceId[arrayLen].Split('&amp;'); serial = serialArray[0]; return serial; } private string getValueInQuotes(string inValue) { string parsedValue = ""; int posFoundStart = 0; int posFoundEnd = 0; posFoundStart = inValue.IndexOf("\""); posFoundEnd = inValue.IndexOf("\"", posFoundStart + 1); parsedValue = inValue.Substring(posFoundStart + 1, (posFoundEnd - posFoundStart) - 1); return parsedValue; } } } </code></pre> <p>Source: <a href="http://www.cfdan.com/posts/Retrieving_Non-Volatile_USB_Serial_Number_Using_C_Sharp.cfm" rel="noreferrer">http://www.cfdan.com/posts/Retrieving_Non-Volatile_USB_Serial_Number_Using_C_Sharp.cfm</a></p>
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    1. COYes, it's available all the way back to the 1.1 framework, according to MSDN: http://msdn.microsoft.com/en-us/library/system.management.aspx
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