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copied!<p>The problem in your code is that <code>prmInstance</code> variable and <code>initializerRegion</code> function declared in global execution context. So the last control overrides <code>initializerRegion</code> function definition. To fix this you may wrap all your code in self called function like below:</p> <pre><code>(function () { var prmInstance = Sys.WebForms.PageRequestManager.getInstance(); prmInstance.add_endRequest(function () { //you need to re-bind your jquery events here initializerRegion(); }); var initializerRegion = function () { $('#<%= autoRegion.ClientID %>').autocomplete({ source: function (request, response) { //...... }, //...... }); $(function () { initializerRegion(); }); })(); </code></pre> <p>This code works well for me:</p> <p>ascx:</p> <pre><code><script type="text/javascript"> (function () { var prmInstance = Sys.WebForms.PageRequestManager.getInstance(); prmInstance.add_endRequest(function () { initialize(); }); var initialize = function () { $("#<%= TextBox1.ClientID %>").on("keyup", function () { alert(this.value); }); }; $(function () { initialize(); }); })(); </script> <asp:TextBox runat="server" ID="TextBox1" /> </code></pre> <p>aspx:</p> <pre><code><asp:ScriptManager runat="server" /> <asp:UpdatePanel runat="server" UpdateMode="Conditional"> <ContentTemplate> <uc:WebUserControl2 runat="server" ID="ucWebUserControl2" /> <asp:Button Text="Click Me" runat="server" /> </ContentTemplate> </asp:UpdatePanel> <hr /> <asp:UpdatePanel runat="server" UpdateMode="Conditional"> <ContentTemplate> <uc:WebUserControl2 runat="server" ID="WebUserControl1" /> <asp:Button Text="Click Me" runat="server" /> </ContentTemplate> </asp:UpdatePanel> </code></pre>

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